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. 2017 Oct 15;28(21):2887–2903. doi: 10.1091/mbc.E17-01-0082

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© 2017 Bondu et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — P2Y₂R expression in various cell lines. (A) Plot of P2ry2 mRNA expression in cell lines used in this study, namely P2Y₂R-null wild type astrocytoma cells (WT1321N1), 1321N1 cells stably expressing an Arg95-Gly96-Glu97 (RGE) mutation of the Arg95-Gly96-Asp97 (RGD) sequence in the P2Y₂R (^RGEP2Y₂R) and 1321N1 cells expressing wild-type P2Y₂R (^RGDP2Y₂R), CHO-K1 and telomerase-immortalized human microvascular endothelium cell line (TIME). RNA was extracted from 150,000 cells in duplicate wells with RNeasy Qiagen kit. Quantitative RT–PCR was performed in triplicate for each well by Taqman assay as described under Materials and Methods. (B) Plot of P2ry2 mRNA knockdown in CHO-K1 stably transfected with α_IIbβ₃–integrin measured 24 h after siRNA transfection, **p < 0.05.