Table 1. The sequence close to dif is specifically lost in recB mutants.
| Genotype | % cells with 0 focus | initial events | transmitted* | |
|---|---|---|---|---|
| ydeV::parSpMT1 | yoaC::parSpMT1 | ydeV::parSpMT1 | ||
| wild-type | 0.6 ± 0.2 | 0.6 ± 0.3 | ||
| recB | 32 ± 1.5 | 7.9 ± 1 | 17.7% (350) | 74.5% |
| recC | 30.6 ± 1.6 | 7.8 ± 1.6 | ||
| recD | 0.63 ± 0.74 | 0.34 ± 0.37 | ||
| tus | 0.8 ± 0.24 | 2.5 ± 0.3 | ||
| tus recB | 36.7 ± 2.1 | 13.1 ± 0.8 | 15.8% (240) | 87.1% |
| pspE::TerB | 0.3 ± 0.3 | |||
| pspE::TerB recB | 48 ± 7 | 8.8 ± 7 | 25.3% (383) | 80.65% |
| pspE::TerB tus | 0.8 ± 0.06 | |||
| pspE::TerB tus recB | 35.1 ± 5.6 | |||
| xerC | 14.8 ± 1 | 1.7 ± 1.4 | ||
| xerC recB | 40.4 ± 0.9 | 14 ± 2.6 | ||
| dif | 15.2 ± 1.8 | |||
| dif recB | 41.5 ± 2 | 17.5% (498) | 83.9% | |
| dif hipA | 15.7 ± 2.5 | 3.6 | ||
| dif hipA recB | 64.6 ± 7 | 40 ± 3.2 | ||
| ftsKATPase | 20.6 ± 0.4 | 5.9 ± 0.6 | ||
| ftsKATPase recB | 54.7 ± 0.1 | 14 ± 0.08 | ||
| ftsKΔCter | 25.1 ± 1.9 | 4.5 ± 2.3 | ||
| ftsKΔCter recB | 54.4 ± 1.2 | 15.9 ± 3.1 | 15.8% (303) | 82.8% |
| ftsKATPase tus | 27.3 ± 0.7 | |||
| ftsKATPase tus recB | 49.8 ± 1.2 | |||
| recB [pET28] | 34.2 ± 2.4 | |||
| recB [pET-parC-TD] | 32.4 ± 4.7 | |||
| endA recBa | 35.2 ± 3.7 | |||
| InvT3 | 4.9 ± 2.1 | |||
| InvT3 recB | 38.4 ± 3.6 | |||
| InvT2 | 8.7 ± 3.7 | |||
| InvT2 recB | 43.5 ± 3.4 | |||
(a) experiment in the endA recB mutant was realized with the ParB protein expressed from plasmid pFHC2973 [42].
Examples of time lapse experiments are shown in Figs 2, 3 and 6 and in S1–S7 Videos. For analysis, the number of divisions providing two foci-containing cells and the number of first divisions providing one focus-containing and one focus-less cells were manually counted (see Fig 2E and Material and Methods). Initial events shows the percentage of divisions that lead to one cell with a focus and one cell without focus, not counting the ones that follow a first event. The total number of counted divisions is between parentheses. Results are the sum of two experiments.
(*) The transmission to progeny was calculated on the events for which at least one additional division could be observed (50–80% of the initial events). Only fully hereditary events are counted, about 10–15% were hereditary but with an interruption of transmission for one generation and are not included here. Few (5–10%) occurred once with no evidence for transmission.