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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Dev Biol. 2017 Apr 12;432(1):72–85. doi: 10.1016/j.ydbio.2017.04.002

Fig. 3. Normal ventral patterning of the Spopl mutant and Spopl;Spop double mutant spinal cords.

Fig. 3

(A–A″) Lateral views of E10.5 control (A), Spop mutant (A′) and Spopl;Spop double mutant (A″) embryos. (B–F″) Immunofluorescent images of transverse sections of E10.5 control (n=4 embryos), Spopl mutant (n=4 embryos) and Spopl;Spop double mutant (n=4 embryos) spinal cords at the forelimb level with indicated antibodies are shown. The spinal cords are outlined with dash lines. Brackets indicate the expression domains. The span of each domain along the D/V axis is quantified and shown on the right. Relative D/V position is shown as the distance to the ventral-most point of the spinal cord as a percentage of the entire D/V span of the spinal cord. Student’s t-test suggests no significant difference between Spopl;Spop double mutant, Spopl mutant and control groups. The morphology and spinal cord neural patterning of both Spopl mutant and Spopl;Spop double mutant resemble wild type.