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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Dev Biol. 2017 Apr 12;432(1):72–85. doi: 10.1016/j.ydbio.2017.04.002

Fig. 5. Spop;Gli3 double mutants resembled Gli3 mutants in ventral spinal cord patterning.

Fig. 5

(A–A″) Lateral views of E10.5 control (A), Gli3 mutant (A′) and Spop;Gli3 double mutant (A″) embryos. Arrows in A′ and A″ indicate exencephaly. (B–F″) Immunofluorescent images of transverse sections of E10.5 spinal cords in Gli3 mutant (n=4 embryos) and Spop;Gli3 double mutant (n=5 embryos) resembled those in the control (n=5 embryos) at the forelimb level with indicated antibodies. The spinal cords are outlined with dash lines. Brackets indicate the expression domains. The span of each domain is quantified and shown on the right. Relative D/V position is shown as the distance to the ventral-most point of the spinal cord as a percentage of the entire D/V span of the spinal cord. Student’s t-test suggests no significant difference between Spop;Gli3 double mutant, Gli3 mutant and control groups.