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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Dev Biol. 2017 Apr 12;432(1):72–85. doi: 10.1016/j.ydbio.2017.04.002

Fig. 7. Loss of Spop exacerbated the ventralization of the Sufu mutant spinal cord.

Fig. 7

(A–C) Lateral views of E9.5 wild type (A), Sufu mutant (A′) and Spop;Sufu double mutant (A″) embryos. Arrows in B and C indicate exencephaly; arrowheads indicate spina bifida. (B–F″) Immunofluorescent images of transverse sections of E9.5 spinal cords at the thoracic level. The spinal cords are outlined with dash lines. Brackets indicate the expression domains. (B–C″) Foxa2 and Nkx2.2 were expressed in the ventral-most region of wild type but expanded to the dorsal region in the Sufu mutant and Spop;Sufu double mutant spinal cords. (D–D″) Nkx6.1 expression domain was expanded to the dorsal region in both Sufu mutant and Spop;Sufu double mutant spinal cords. (E–E″) Olig2 expression was shifted to the dorsal region in Sufu mutant and even more dorsally shifted in Spop;Sufu double mutant spinal cords. (F–F″) Pax6 expression domain was shifted dorsally in Sufu mutant and was absent in Spop;Sufu double mutant spinal cord. n=2 embryos for each genotype were analyzed. The span of each domain is quantified and shown on the right. Relative D/V position is shown as the distance to the ventral-most point of the spinal cord as a percentage of the entire D/V span of the spinal cord.