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. Author manuscript; available in PMC: 2018 Oct 11.
Published in final edited form as: Cell Host Microbe. 2017 Sep 21;22(4):531–542.e8. doi: 10.1016/j.chom.2017.08.009

Figure 1. High Mn diet increases lethality and heart bacterial burdens from S. aureus systemic infection.

Figure 1

WT mice were fed modified diets and infected with S. aureus for four days (A–J) or 18 hours (K–L). (A–B) Mn concentrations in (A) heart homogenates and (B) liver homogenates were measured by ICP-MS. N = 3. (C) Survival was monitored. Low Mn N = 10; control N = 29; high Mn N = 34. (D) CFU present in heart homogenates. For (D–E) and (H), N = 16 (control), 11 (high Mn). (E) Enumeration of heart abscesses. (F) Heart appearance. Arrowheads indicate abscesses. (G) CFU present in kidney homogenates. N = 11 (control); N = 9 (high Mn). (H) CFU present in liver homogenates. (I) CFU present in liver homogenates. N = 18 (control); N = 19 (low Mn). (J) CFU present in heart homogenates from mice fed modified Zn diets. N = 7 (high Zn, low Zn), 9 (control). (K–L) Flow cytometry quantification of immune cells in the heart. Previous gate: live cells. N = 7 (infected), N = 3 (mock). (K) Neutrophils, Ly6G+ CD11b+. (L) Macrophages, F4/80+. (A–B) Bars depict mean and SEM. (D–E, G–L) Bars depict median and interquartile range. ns = not significant. *P < 0.05. ** P < 0.01. *** P < 0.001 by log-rank test (C), Mann-Whitney test (D, E, G–I) or one-way analysis of variance (ANOVA) with Dunnett’s multiple comparisons test (A–B) or Tukey’s multiple comparisons test (J–L). See also Figure S1.