Skip to main content
. Author manuscript; available in PMC: 2018 Oct 11.
Published in final edited form as: Cell Host Microbe. 2017 Sep 21;22(4):531–542.e8. doi: 10.1016/j.chom.2017.08.009

Figure 2. Dietary Mn does not alter the gut microbiota or impact infection with Clostridium difficile or Acinetobacter baumannii.

Figure 2

(A–C) Microbiota analysis during a 4-week time course of dietary Mn manipulation. (A) Nonmetric multidimensional scaling (NMDS) ordination showing gut microbiota β-diversity. Distances were measured using the Yue and Clayton index of dissimilarity (θYC). No significant differences were observed between groups (Analysis of Molecular Variance; AMOVA) (P > 0.05). (B) Inverse Simpson’s diversity for each diet. (C) Species richness measured by Sobs (number of observed OTUs) for each diet. (D) Blinded histological scores from ceca were determined 4 days post-infection with C. difficile. Error bars represent standard deviation. (E) CFU recovered from lungs 36 hours following intranasal inoculation with A. baumannii. (F–G) CFU recovered from (F) heart and (G) liver, lungs, spleen and kidney 24 hours following intravenous inoculation with A. baumannii. (E–G) Bars indicate median +/− interquartile range. ns = not significant by ANOVA with Tukey’s multiple comparison’s test (E) or Mann-Whitney (F, G).