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. Author manuscript; available in PMC: 2018 Oct 11.
Published in final edited form as: Cell Host Microbe. 2017 Sep 21;22(4):531–542.e8. doi: 10.1016/j.chom.2017.08.009

Figure 4. Loss of calprotectin does not impact Mn bioavailability in the heart.

Figure 4

(A) Heart bacterial burdens from WT and S100a9−/− mice provided normal chow and infected for 4 days with S. aureus WT or ΔmntH/C. N = 15 (S100a9−/− ΔmntH/C), N = 14 (all other groups). (B) Mn concentrations in heart homogenates, measured by ICP-MS. Hearts are from infected WT or S100a9−/− (a9−/−) mice fed high Mn, control, or low Mn diet. N = 3. (C) S. aureus growth on RPMI agar containing 10 μM TPEN spotted with CP or MnCl2 sources as labeled. Number of bacteria plated: high density = 2 × 108 CFU, low density = 2 × 107 CFU. Red arrow = growth inhibition, black arrow = growth enhancement. (A) Bars depict median and interquartile range. (B) Bars depict mean and SEM. *** P < 0.001, ns = not significant by ANOVA with Sidak’s multiple comparisons test. See also Figure S3.