Figure 6. Excess dietary Mn is bioavailable to S. aureus in the heart.
WT mice were fed high Mn or control diet and infected mock-infected with PBS (A) or infected with S. aureus WT (B) for 4 days. The arrangement and relative concentrations of Mn and Ca in heart sections were assessed by LA-ICP-MS. Left, H&E stained serial sections. Scale bar is 1 mm. (C) Heart bacterial burdens from infection with WT or ΔmntH/C. N = 14 (Control WT, high Mn ΔmntH/C); 15 (control ΔmntH/C, high Mn WT). Bars are median +/− interquartile range. *** P < 0.001 by ANOVA with Tukey’s multiple comparisons test. See also Figure S2.
