Fig. 1.

Activation of RhoA and Ezrin in the vaginal tissue.

(A) Protein expression levels of p-RhoA, RhoA, and Actin in PRE, PAV, and PON were assessed by Western blotting. Shown is the average of three independent experiments, each performed in triplicate. Total RhoA and Actin was represented as the amount of the loaded protein. (B) Expression levels of p-Ezrin, Ezrin, and Actin in PRE, PAV, and PON were assessed by Western blotting. Data represent the average of three independent experiments, each performed in triplicate. Total Ezrin and Actin was represented as the amount of the loaded protein. (C) Expression levels of mRNA of total RhoA and Ezrin in PRE, PAV, and PON were assessed by RT-PCR.