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. 2017 Oct 12;7:13026. doi: 10.1038/s41598-017-13432-x

Figure 3.

Figure 3

TFGloxP/loxP; MIP-Cre mice showed reduced β-cell mass as a consequence of diminished β-cell proliferation. (a) Pancreatic sections from 9-week-old mice were immunostained with antibodies against insulin (red) and glucagon (green). scale bars: 500 μm (left panels), 50 μm (right panels). (b) Box plot and histogram for β-cell area per islet (f/f: 249 islets from 8 mice, KO: 269 islets from 10 mice, Mann-Whitney U test, **P < 0.01). (c) Percentage of β-cell area over total pancreatic area. (d) Immunostaining for Ki-67 (green) and insulin (red). White arrowheads indicate Ki-67 positive β-cells. scale bars: 100 μm. (e) Percentage of Ki-67 positive β-cells (f/f: 41 positive cells among 6022 β-cells, KO: 24 positive cells among 5006 β-cells). 9-week-old mice, n = 8–10 (b–e), *P < 0.05, **P < 0.01.