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. 2017 Oct 12;7:13079. doi: 10.1038/s41598-017-12960-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Response of the haMSC exposed to one pulse of 100 μs in DMEM (panels A and C) or in SMEM-EGTA (panels B and D). (A,B) Percentage of cells displaying calcium peaks. (C and D): Mean amplitude of the calcium peaks. E⁰, E⁵⁰, and E¹⁰⁰ are the values of the electric field amplitudes needed to respectively start to permeabilize the cells, and for the permeabilization of 50% and 100% of the cells. The gray curves in (B,D) represent the curves (A,C), respectively. The inset in (D) represent a magnification of the transition between the fast and the slow increase of the amplitude of the calcium peaks. (n = 3 to 7 independent experiments).