Fig. 3.

Defective response of Prmt1-deficient B cells to in vitro stimulation. Control (+/+) and Prmt1 ^f/f CD23Cre (f/f) splenic B cells, labelled with CTV, were activated with a–d CD40L + IL-4 + IL-5 or e–g LPS (25 μg ml⁻¹) for the indicated times. a, e Cultured cells were monitored for ASC differentiation with anti-CD138 antibody (top panels) and extent of cell division with CTV dilution (bottom panels). Percentages are the proportion of viable cells within indicated regions. b, f Frequency of live, CD138⁺ ASC at 4 days after activation. c, g Number of viable cells in cultures after stimulation for the indicated times. d Frequency of CD138⁺ cells within the indicated cell divisions of CD40L + IL-4 + IL-5 stimulated control (+/+) and Prmt1 ^f/f CD23Cre (f/f) splenic B cells at day 4. *P ≤ 0.05 **P ≤ 0.01, ***P ≤ 0.001 (unpaired t-test). One representative experiment of three, with three biological replicates, is shown. (Mean and s.e.m in b–d, f, g). Flow cytometry gating strategies for this figure are shown in Supplementary Fig. 7