Fig. 6.

PRMT1 is required to mount a recall immune response. a–c Irradiation chimeras were generated with 80% of µMT and either 20% of Prmt1 ^+/+ Rosa26CreERT2 (G1;+/+) or 20% Prmt1 ^f/f Rosa26CreERT2 (G2; f/f) BM cells. Nine weeks post BM reconstitution; mice were immunized with NP-KLH in alum. Nine weeks after immunization mice were treated for 3 days with single doses of Tamoxifen to induce Prmt1 deletion and then boosted with NP-KLH in PBS, injected i.p. a Representative flow cytometry plots of splenocytes 5 days after NP-KLH boost are shown. Frequencies of NP⁺IgG1⁺ (left) and total GC (B220⁺Fas⁺PNA⁺) (right) among B220⁺IgM⁻IgD⁻Gr1⁻ cells and B220⁺ cells, respectively, are indicated. Numbers represent percentages of the displayed events within indicated gates. b, c Average numbers of NP⁺IgG1⁺ B-cells, GC B cells, total splenocytes per spleen and per femur are shown in the graphs. *P ≤ 0.05; ns = not significant (unpaired t-test). Mean and s.e.m. (b, c). The experiment comprised five mice per group. Flow cytometry gating strategies for this figure are shown in Supplementary Fig. 10