Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Oct 12;7:13090. doi: 10.1038/s41598-017-12998-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Generation of Rosa26^{Flox-Stop-Flox-IRES-Ncr1} mouse and crossing with CD2^cre and LCK^cre mice. (A) Schematic representation of the Rosa locus-targeting vector. (B) Clones of ES cells were tested for Ncr1 mRNA expression following cre recombinase treatment. Ncr1 relative expression, compared to control ES cells is presented. The experiment combines data from three independent experiments. Values are shown as mean ± SEM. *P < 0.05. (C) FACS staining of ES cells, untreated, or following cre treatment. Gray histograms represent background control. Black histograms represent specific staining. Each FACS plot is representative of at least three independent experiments. (D) Chimeric mice were crossed to WT C57BL/6 mice and the progeny were genotyped using specific primers. DNA samples run on gel are presented. Specific bands sized 535 bp signify the inserted Ncr1 gene. The figure represents the four founder breeders. Contrasts were adjusted for clarity. (E,F) Rosa26^{Flox-Stop-Flox-IRES-Ncr1} mice were bred with CD2^cre and LCK^cre mice. Offspring were denoted CD2^cre Ncr1^OE and LCK^cre Ncr1^OE respectively. (E) FACS plots depicting CD3 and Ncr1 expression in the transgenic mice, compared to a littermate. (F) FACS plots depicting CD3, CD4 and CD8, and GFP expression in the transgenic mice, compared to a littermate. The plots are representative of five independent stainings. Percentages are indicated.