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. 2017 Oct 12;7:13021. doi: 10.1038/s41598-017-13398-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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ABHD5 silencing reduces oxygen consumption and downregulates the expression of RCC I and III. Basal extracellular acidification rate (EACR) (A) and oxygen consumption rate (OCR) (B) of LNCaP cells measured with a Seahorse metabolic flux analyzer. Data are presented as mean ± SEM (n = 3) of experiments performed in triplicate. Two-tailed t-test, *P < 0.05, ***P < 0.001. (C) Protein levels of respiratory chain complexes of LNCaP cells visualized with Total OXPHOS antibody cocktail in Western Blotting.