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. 2017 Oct 12;7:13021. doi: 10.1038/s41598-017-13398-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of ectopic ABHD5 overexpression on the proliferation and invasion of C4-2B cells. (A) Expression level of ABHD5-Flag, E-Cadherin, Snail, HK2, PFKP and β-actin proteins in C4-2B/ABHD5 cells treated with or without doxycycline (dox). (B) Level of intracellular TAG content in C4-2B/ABHD5 cells treated with or without dox. Two-tailed t-test, *P < 0.05. (C) Repression of cell invasion by ABHD5. A representative image of Transwell invasion assay is shown for each group, respectively, on the left. Numbers of invasive cell are plotted on the right. Data are presented as mean ± SEM (n = 3) of experiments performed in triplicate. Two-tailed t-test, ***P < 0.001. (D) Repression of colony formation by ABHD5. A representative image of soft agar assay is shown for each group, respectively, on the left. Number of colonies is plotted on the right. Data are presented as mean ± SEM (n = 3) of experiments performed in triplicate. Two-tailed t-test, ***P < 0.001. (E) Repression of cell proliferation by ABHD5. Growth curve is plotted for C4-2B cells grown in the presence or absence of doxycycline. Data are presented as mean ± SEM (n = 3) of experiments performed in triplicate. Two-way ANOVA test, *P < 0.05.