Fig. 1.

Distinct RNA groups are targeted at protrusions through APC-dependent and APC-independent pathways. a Pie chart of non-localized or protrusion (Ps)-enriched RNAs in control knockdown (KD) cells from 4 replicate experiments. Cutoffs for Ps-enrichment were set at FC > 2 and p-value < 0.05. b Cumulative fraction plot of log2 fold-change differences between control and APC knockdown cells, for RNAs non-localized or localized in protrusions of control cells. c For all Ps-localized RNAs of control cells, the log₂ differences in FC values between control and APC knockdown cells were plotted against the corresponding p-values. Applying the cutoffs marked by the red lines (see Supplementary Data 1 for details), Ps-localized RNAs were distinguished into APC-dependent and APC-independent groups. d Categories of molecular functions, derived through IPA analysis, significantly represented in APC-dependent and APC-independent RNA groups. The identities of the RNAs included in the groups are listed. e Control or APC knockdown cells were fractionated into Ps and cell body (CB) fractions. The indicated RNAs were detected through nanoString analysis to calculate Ps/CB enrichment ratios (n = 3; error bars: standard error). p-value: **< 0.0001, *< 0.04 by analysis of variance with Bonferroni’s multiple comparisons test against the corresponding control