Fig. 2.

APC-dependent and APC-independent RNAs are enriched in different types of protrusions, associated with high or low levels of contractility. a NIH/3T3 fibroblast cells were plated on microporous filters containing pores of 3 or 0.4 μm in diameter. The cells were induced to extend protrusions by addition of LPA in the bottom chamber and were subsequently fixed and stained to detect actin (green), tubulin (red), and nuclei (DAPI, blue). Confocal xy-slices through the cell body or protrusions, or xz-orthogonal views are shown. Scale bars: 4 μm. b Ps and CB fractions were isolated from cells extending protrusions through 3 or 0.4 μm pores. The indicated RNAs were detected through nanoString analysis to calculate Ps/CB enrichment ratios (n = 3; error bars: standard error). c Ps and CB fractions, of cells extending protrusions through 3 or 0.4 μm pores, were analyzed by western blot to detect the indicated proteins. Results are representative of two independent repeats