Fig. 5.

Detyrosinated microtubules are necessary and sufficient for the peripheral localization of Ddr2 RNA. a Schematic depicting the microtubule detyrosination cycle. Indicated is the C-terminal tyrosine residue removed by an unknown carboxypeptidase. The effects of parthenolide treatment or knockdown of tubulin tyrosine ligase (TTL) are also shown. b FISH analysis of control or parthenolide treated cells plated on 5 kPa substrates. Representative Ddr2 RNA distributions and PDI values of Ddr2 and polyA RNA are shown. c FISH analysis of control or TTL knockdown cells plated on 1 kPa substrates. Representative Ddr2 RNA distributions and PDI values of Ddr2 and polyA RNA are shown. For PDI quantifications, bars represent the mean with 95% confidence interval. Points indicate individual cells analyzed in at least two or more independent experiments. p-values: ***< 0.001 by analysis of variance with Bonferroni’s multiple comparisons test. Scale bars: 10 μm