Table 2.
Summary of electrophysiological recordings
| GluA2iQ constructs | Auxiliary subunit constructs | n | Holding potential (mV) | τ (weighted) (ms) | SS/P | ||
|---|---|---|---|---|---|---|---|
| Data set 1 (dual expression without using intein) | |||||||
| GluA2iQ | 10 | −70 | 5.73 ± 0.150 | 0.010 ± 0.0031 | |||
| GluA2iQ | CNIH3 | 6 | −70 | 35.9 ± 2.69 | 0.13 ± 0.021 | ||
| GluA2iQ‐C528L | 7 | −70 | 4.67 ± 0.173 | 0.062 ± 0.049 | |||
| GluA2iQ‐C528L | CNIH3 | 7 | −70 | 11.1 ± 1.15 | 0.037 ± 0.0070 | ||
| GluA2iQ‐L789F | 6 | −70 | 4.70 ± 0.224 | 0.013 ± 0.0030 | |||
| GluA2iQ‐L789F | CNIH3 | 6 | −70 | 4.43 ± 0.211 | 0.025 ± 0.0053 | ||
| GluA2iQ‐A793F | 6 | −70 | 9.13 ± 1.10 | 0.030 ± 0.0066 | |||
| GluA2iQ‐A793F | CNIH3 | 8 | −70 | 34.1 ± 5.10 | 0.44 ± 0.025 | ||
| GluA2iQ‐A793L | 7 | −70 | 5.17 ± 0.403 | 0.036 ± 0.015 | |||
| GluA2iQ‐A793L | CNIH3 | 5 | −70 | 5.05 ± 0.832 | 0.060 ± 0.017 | ||
| GluA2iQ‐A793G | 7 | −70 | 9.00 ± 1.42 | 0.10 ± 0.025 | |||
| GluA2iQ‐A793G | CNIH3 | 5 | −70 | 8.92 ± 0.711 | 0.066 ± 0.019 | ||
| GluA2iQ‐C528L‐A793F | 8 | −70 | 6.23 ± 0.444 | 0.069 ± 0.051 | |||
| GluA2iQ‐C528L‐A793F | CNIH3 | 5 | −70 | 15.2 ± 1.94 | 0.12 ± 0.028 | ||
| Data set 2 (intein trans spliced) | |||||||
| GluA2iQ | 11 | −70 | 4.87 ± 0.312 | 0.012 ± 0.0017 | |||
| GluA2iQ | Stg | 10 | −70 | 14.5 ± 0.707 | 0.20 ± 0.018 | ||
| GluA2iQ(DDD) | Stg | 15 | −70 | 12.5 ± 0.806 | 0.11 ± 0.011 | ||
| GluA2iQ(AGA) | Stg | 11 | −70 | 12.0 ± 0.934 | 0.17 ± 0.029 | ||
| GluA2iQ(EGE) | Stg | 5 | −70 | 8.85 ± 0.661 | 0.10 ± 0.0095 | ||
| Data set 3 (intein trans spliced) | |||||||
| GluA2iQ | 8 | −60 | 5.78 ± 0.520 | 0.014 ± 0.0028 | |||
| GluA2iQ | Stg | 10 | −60 | 15.0 ± 1.18 | 0.19 ± 0.020 | ||
| GluA2iQ‐C528L | 7 | −60 | 5.87 ± 0.219 | 0.056 ± 0.0081 | |||
| GluA2iQ‐C528L | Stg | 10 | −60 | 9.20 ± 0.973 | 0.083 ± 0.016 | ||
| GluA2iQ‐L789F | 11 | −60 | 4.25 ± 0.248 | 0.034 ± 0.0060 | |||
| GluA2iQ‐L789F | Stg | 8 | −60 | 6.71 ± 0.356 | 0.022 ± 0.0019 | ||
| GluA2iQ‐A793F | 13 | −60 | 7.62 ± 0.427 | 0.053 ± 0.011 | |||
| GluA2iQ‐A793F | Stg | 12 | −60 | 10.3 ± 0.834 | 0.084 ± 0.015 | ||
| GluA2iQ‐G804A | 7 | −60 | 5.15 ± 0.254 | 0.040 ± 0.010 | |||
| GluA2iQ‐G804A | Stg | 16 | −60 | 16.4 ± 1.29 | 0.26 ± 0.026 | ||
| GluA2iQ‐M807L | 6 | −60 | 6.05 ± 0.837 | 0.057 ± 0.027 | |||
| GluA2iQ‐M807L | Stg | 10 | −60 | 12.5 ± 1.03 | 0.14 ± 0.019 | ||
| GluA2iQ‐L808F | 8 | −60 | 5.64 ± 0.204 | 0.019 ± 0.0025 | |||
| GluA2iQ‐L808F | Stg | 8 | −60 | 12.3 ± 1.68 | 0.20 ± 0.031 | ||
| Data set 4 (intein trans spliced) | |||||||
| GluA2iQ | Stg | 10 | −60 | 12.9 ± 1.08 | 0.17 ± 0.025 | ||
| GluA2iQ | Stg‐KK52EE | 7 | −60 | 28.6 ± 5.31 | 0.45 ± 0.040 | ||
| GluA2iQ | Stg‐KK53Enull | 7 | −60 | 23.6 ± 2.88 | 0.48 ± 0.036 | ||
| GluA2iQ | Stg‐K53null | 9 | −60 | 14.9 ± 1.14 | 0.21 ± 0.022 | ||
| GluA2iQ | Stg‐KK52AA | 6 | −60 | 15.9 ± 1.50 | 0.31 ± 0.027 | ||
| GluA2iQ | Stg‐E70K | 5 | −60 | 14.4 ± 1.24 | 0.11 ± 0.015 | ||
| GluA2iQ | Stg‐ED84RK | 7 | −60 | 15.5 ± 2.52 | 0.17 ± 0.036 | ||
| GluA2iQ | Stg‐DAD86KAK | 6 | −60 | 15.7 ± 0.581 | 0.22 ± 0.022 | ||
| GluA2iQ | Stg‐EAD90KAK | 6 | −60 | 22.4 ± 3.04 | 0.47 ± 0.077 | ||
| Data set 5 (intein trans spliced) | |||||||
| GluA2iQ | Stg | 20 | −60 | 13.3 ± 0.572 | 0.20 ± 0.014 | ||
| GluA2iQ | Stg‐KK52EE | 19 | −60 | 21.1 ± 1.42 | 0.43 ± 0.013 | ||
| GluA2iQ | Stg‐KK53Enull | 12 | −60 | 20.6 ± 1.75 | 0.42 ± 0.018 | ||
| GluA2iQ‐C528L | Stg | 16 | −60 | 8.82 ± 0.747 | 0.11 ± 0.020 | ||
| GluA2iQ‐C528L | Stg‐KK52EE | 15 | −60 | 13.0 ± 1.32 | 0.23 ± 0.025 | ||
| GluA2iQ‐C528L | Stg‐KK53Enull | 10 | −60 | 15.5 ± 1.90 | 0.29 ± 0.042 | ||
| one‐way ANOVA with Dunnett's post hoc (vs. GluA2iQ+Stg) | |||||||
| Data set 6 (dual expression without using intein) | τ (weighted) | SS/P | |||||
| GluA2iQ | Stg | 9 | −60 | 10.4 ± 0.531 | 0.11 ± 0.010 | ||
| GluA2iQ‐C528L | Stg | 10 | −60 | 6.90 ± 0.409 | 0.059 ± 0.41 | 0.0049 (**) | 0.0571 (n.s.) |
| GluA2iQ‐L789F | Stg | 10 | −60 | 4.72 ± 0.388 | 0.027 ± 0.0038 | 0.0001 (****) | 0.0004 (***) |
| GluA2iQ‐A793F | Stg | 10 | −60 | 6.25 ± 0.526 | 0.099 ± 0.020 | 0.0006 (***) | 0.978 (n.s) |
| GluA2iQ‐G804A | Stg | 8 | −60 | 12.5 ± 0.813 | 0.16 ± 0.016 | 0.24 (n.s.) | 0.2144 (n.s.) |
| GluA2iQ‐M807L | Stg | 10 | −60 | 12.8 ± 1.26 | 0.15 ± 0.021 | 0.111 (n.s.) | 0.35 (n.s.) |
| GluA2iQ‐L808F | Stg | 9 | −60 | 10.7 ± 0.660 | 0.14 ± 0.013 | 0.9997 (n.s.) | 0.7755 (n.s.) |
| Data set 7 (dual expression without using intein) | |||||||
| GluA2iQ | Stg | 9 | −60 | 11.5 ± 0.579 | 0.14 ± 0.015 | ||
| GluA2iQ | Stg‐KK52EE | 8 | −60 | 21.1 ± 1.65 | 0.40 ± 0.022 | 0.0002 (***) | 0.0001 (****) |
| GluA2iQ | Stg‐KK53Enull | 6 | −60 | 15.6 ± 1.02 | 0.34 ± 0.015 | 0.2079 (n.s.) | 0.0001 (****) |
| GluA2iQ | Stg‐K53null | 7 | −60 | 15.3 ± 1.96 | 0.24 ± 0.035 | 0.2164 (n.s.) | 0.0223 (*) |
| GluA2iQ | Stg‐KK52AA | 8 | −60 | 15.1 ± 2.03 | 0.22 ± 0.030 | 0.2713 (n.s.) | 0.0802 (n.s.) |
The experiment data were categorized in seven data sets. Small differences in gating parameters of the same construct (for example GluA2iQ alone) are likely due to uncontrollable small variations in experimental conditions. A holding potential of −60 mV was preferred for experiments using stargazin because the current amplitude was overall greater. Mean ± SEM are shown. Numbers of experimental replicates (outside‐out patches) are indicated by n. No data was re‐used between data sets. Statistical significance was examined within each data set using one‐ or two‐way ANOVA with appropriate post hoc tests for multiple comparisons as descried in the text.