Table 3.
PAXYB1 protein | Predicted function | Mol. mass (kDa) | No. of peptides | No. of unique peptides | Sequence coverage (%) | Protein bands | Previous identification in φKMV | Previous identification in LKD16 | Previous identification in M32 |
---|---|---|---|---|---|---|---|---|---|
ORF16 | hypothetical protein | 13.3 | 4 | 4 | 37.82 | PB16 | − | − | − |
ORF39 | hypothetical protein | 10.5 | 38 | 7 | 81.63 | PB16 | + | + | + |
ORF41 | head-tail connector protein | 56.2 | 89 | 26 | 53.33 | PB7 | + | + | + |
ORF42 | scaffolding protein | 33.2 | 3 | 3 | 13.04 | PB9 | + | − | − |
ORF43 | capsid protein | 37.7 | 670 | 33 | 85.07 | PB8 | + | + | + |
ORF44 | tail tubular protein A | 21.2 | 12 | 6 | 32.61 | PB13 | + | + | + |
ORF45 | tail tubular protein B | 92.0 | 82 | 36 | 46.97 | PB4 | + | + | + |
ORF46 | Internalvirion protein | 18.8 | 21 | 8 | 44.20 | PB14 | − | + | + |
ORF47 | Internalvirion protein | 98.2 | 147 | 49 | 52.00 | PB4 | + | + | + |
ORF48 | Internalvirion protein | 144.0 | 159 | 61 | 47.57 | PB1 | + | + | + |
ORF49 | putative tail fiber protein | 28.4 | 38 | 13 | 52.59 | PB11 | + | + | + |
ORF50 | putative tail fiber protein | 16.8 | 5 | 2 | 21.71 | PB15 | − | − | − |
ORF51 | putative tail fiber protein | 33.2 | 40 | 9 | 28.90 | PB9 | + | + | + |
ORF52 | putative tail fiber protein | 22.6 | 25 | 9 | 48.76 | PB12 | − | + | + |
ORF57 | Rz protein | 12.0 | 2 | 2 | 12.84 | PB16 | − | − | − |
ORF59 | putative structural protein | 10.5 | 93 | 6 | 83.65 | PB16 | + | + | + |
ORF60 | putative structural protein | 6.9 | 8 | 3 | 43.75 | PB17 | + | + | + |
The proteins detected by MS are listed with their predicted functions and molecular mass. Molecular mass was calculated from the gene sequence. The number of identified peptides and unique peptides in each protein and the corresponding protein sequence coverage are also indicated.