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. 2017 Oct 12;7:13055. doi: 10.1038/s41598-017-12915-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Catalytic inactivation of Polη causes similar defects as the deletion of the RAD30 gene. (a) Strains carrying the Polη D30A mutant are sensitive to both UV and 6-AU. Ten fold serial dilutions of the indicated strains were spotted on media containing the indicated concentration of 6-AU, or irradiated with the indicated UV-dose after spotting. (b) Induced level of GAL10 mRNA is decreased in the Polη D30A mutant, as measured by RT-qPCR. The mean value obtained for the wild-type strain was set to 100% and the values obtained with the other strains are shown relative to that. (c) Occupancy of PolII in the Polη-Myc and the D30A Polη-Myc mutant expressing strains was measured with ChIP using anti-Myc antibody as described in Fig. 6d. Mean and standard deviations based on at least 4 experiments are indicated, p-values were calculated by 2-tailed t-test,n.s.: no statistical difference.