Table 2.
Kinetic parameters of rNTP incorporation into DNA and RNA by Polη.
| Primer | Insertion opposite | Incoming riboucleotide | Kcat (min-1) | Km (µM) | Kcat/Km | Relative efficiencya |
|---|---|---|---|---|---|---|
| RNA | T | ATP | 0.2394 ± 0.0065 | 466.4 ± 47.29 | 5.13E-04 | 3.34 |
| RNA | G | CTP | 2.758 ± 0.06217 | 438.3 ± 37.52 | 62.9E-04 | 18.26 |
| RNA | C | GTP | 0.4487 ± 0.01485 | 393.7 ± 52.04 | 11.4E-04 | 30.24 |
| RNA | A | UTP | 0.1032 ± 0.005715 | 423.3 ± 90.45 | 2.43E-04 | n.d |
| DNA | T | ATP | 0.1163 ± 0.009014 | 757.6 ± 160 | 1.53E-04 | |
| DNA | G | CTP | 0.1733 ± 0.007439 | 503.1 ± 68.83 | 3.44E-04 | |
| DNA | C | GTP | 0.01851 ± 0.000891 | 491.2 ± 76.14 | 0.37E-04 | |
| DNA | A | UTP | — | — | — |
Values were obtained from results shown in Fig. 2, and represent the mean and standard error of three experiments. Kinetic parameters were calculated as described in “Materials and Methods”. n.d, not determined (no insertion of UTP into DNA could be detected). Relative efficiency was calculated using the following equation: f ext = (k cat /K m)RNA/(k cat /K m)DNA.