Skip to main content
. 2017 Oct 12;7:13055. doi: 10.1038/s41598-017-12915-1

Table 4.

Kinetic parameters of dNTP or rNTP incorporation by Polη into RNA opposite DNA damages.

Insertion opposite Incoming nucleotide kcat (min−1) Km (µM) kcat/Km nucleotide concentrationa (µM) Relative frequency
oxoG dCTP 1.7018 ± 0.1177 69.85 ± 18.53 24.36 E-03 14 19.4
oxoG rCTP 0.03424 ± 0.00358 973.97 ± 270.44 3.53 E-06 500
TT dimer dATP 4.9105 ± 0.5331 287.12 ± 83.85 17.10 E-03 16 18.6
TT dimer rATP 0.00831 ± 0.00106 1677.9 ± 444.8 4.95 E-06 3000

a In vivo dNTP and rNTP concentrations are according to ref.14.

Values were obtained from results shown in Fig. 4, and represent the mean and standard error of three experiments. Relative frequency was calculated using the formula as for Table 3.