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. 2017 Oct 12;7:13043. doi: 10.1038/s41598-017-13316-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Various concentrations of diphyllin loaded PEG-PLGA nanoparticles were added to fcwf-4 cells in a direct infection (white open bar) or ADE infection (black bars) for 48 hr incubation (NTU156, MOI = 0.0035). The viral titers from the collected culture supernatants were titrated by plaques assays. Viral titers between each diphyllin-treated group and the untreated control group were compared by one-way ANOVA followed by Dunnett’s multiple comparisons test (**p < 0.01). Data in the plot present the mean ± SEM out of three replicates.