Fig. 2. The different assembling structure proteins identified on SDS-PAGE gels after separation by Superdex 200 gel filtration chromatography (inset). M, the protein molecular mass standard; lane 0, the total protein after purification by Ni-NTA His Band Resin; lanes 1–3, correspond to peaks 1–3 represent the larger aggregate, the dimer, and the monomer. Peak 4 was the elution of imidazole.