Fig. 3. The specific immunogenicity of recombinant Nsp7 tested by enzyme-linked immunosorbent assay. (A) Immunoreaction of the recombinant Nsp7 purified through Ni-chelating affinity chromatography with positive sera to porcine reproductive and respiratory syndrome virus (PRRSV) strain BJ-4 and HN07-1. (B–D) Immunoreaction of larger aggregate, dimer, and monomer separated by a gel filtration chromatography with positive sera to PRRSV strain BJ-4 and HN07-1.
