Fig. 1. Transcriptional expression profiles of Wnt signaling-related genes in canine mammary tumors (CMTs). Total RNA extracted from CMT samples or normal mammary gland tissues were reverse transcribed into cDNA for quantitative PCR analysis with an RT² Profiler PCR Array system. The transcriptional level of these genes are expressed as fold changes. (A and B) Wnt and Wnt antagonist genes. (C) Frizzled receptors and co-receptor. (D) β-catenin and destruction complex-related factors. (E) Transcription factors. (F) Target genes. (G) Upregulated genes in 4 groups of different CMT subtypes. Benign, benign tumor; LM, low malignant tumor; HM, high malignant tumor; CC, complex carcinoma; CMM, carcinoma and malignant myoepithelioma; EC, epithelial carcinoma (malignant tumors with generally only one component (epithelial)). Significant differences expressed as ^*p < 0.05 while highly significant differences are expressed as ^**p < 0.01. Asterisk(s) directly above the error bar of each column indicates a comparison between the indicated group and the benign group; while the Asterisk(s) above the drawn lines means the comparison between the tumor groups indicated by the lines.