Fig. 4. Expression of β-catenin, TCF7, LEF1 and cyclin D1 in CMTs analyzed by immunoblotting. The samples include 1 normal canine mammary tissue (N), 2 complex adenomas (33 and 35), 2 simple adenomas (4 and 2), 1 complex carcinoma (43), 2 simple tubulo-papillary carcinomas (78, Grade I; 80, Grade II), 2 intraductal papillary carcinomas (86, Grade I; 84, Grade II) and 2 solid carcinomas (93, Grade II; 92, Grade III). Primary antibodies against β-catenin, TCF7, LEF1 and cyclin D1 were used for detection. β-actin was used as the reference protein. The malignant grades of the CMTs are marked as I, II, and III on the top of the image.
