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. 2017 Sep 19;10(9):1083–1093. doi: 10.14202/vetworld.2017.1083-1093

Table-3.

Biochemical characteristics of the suspected E. coli isolates using API20E system.

Type of samples Number of samples API 20E results Number of recovered isolates Recovery rate (%)

ONPG ADH LDC ODC CIT H2S URE TDA IND VP GEL GLU MAN INO SOR RHA SAC MEL AMY ARA OX
Apparently healthy 11 + - + + - - - - + - - + + - + + + + - + - 4 8 73
+ - + - - - - - + - + + - + + - + - + - 2
+ + + + - - - - + - - + + - + + + + - + - 2
Diseased 17 + - + + - - - - + - - + + - + + + + - + - 5 14 82
+ - + - - - - - + - - + + - + + - + - + - 3
+ + + + - - - - + - - + + - + + + + - + - 4
+ - - - - - - - + - - + + - + + + + - + - 2
Freshly dead 14 + - + + - - - - + - - + + - + + + + - + - 3 11 79
+ - + - - - - - + - - + + - + + - + - + - 2
+ + + + - - - - + - - + + - + + + + - + - 3
+ - - - - - - - + - - + + - + + + + - + - 3
Total 42 33

E. coli=Escherichia coli, ONPG=Ortho nitro phenyl-βD-galactopyranosidase, ADH=Arginine dihydrolase, LDC=Lysine decarboxylase, ODC=Ornithine decarboxylase, CIT=Citrate utilization, H2S=Hydrogen sulfide, URE=Urease, TDA=Tryptophan deaminase, IND=Indole, VP=Voges Proskauer, GEL=Gelatinase, GLU=Glucose (fermentation/oxidation), MAN=Mannitol (fermentation/oxidation), INO=Inositol (fermentation/oxidation), SOR=Sorbitol (fermentation/oxidation), RHA=Rhamnose (fermentation/oxidation), SAC=Saccharose (fermentation/oxidation), MEL=Melibiose (fermentation/oxidation), AMY=Amygdalin (fermentation/oxidation), ARA=Arabinose (fermentation/oxidation), OX=Oxidase