Fig. 3. Ornithine decarboxylase (ODC) siRNA exacervates the consequences of ischemia and reperfusion injury (IRI). Mice were subjected to 30 minutes of bilateral kidney ischemia or sham operation followed by 6 hours or 24 hours of reperfusion. ODC siRNA or control siRNA was injected at 48 hours and 24 hours before ischemia. n=9 mice in each group. (A) DNA nitration represented by 8-nitroguanine in kidneys. (B) Poly(ADP-ribose) polymerase 1 (PARP1) activity in kidneys. (C) Tubular damage in the outer medulla represented by periodic acid-Schiff (PAS) stain in kidney sections of mice. Scale bars=50 µm. (D) Tubular injury score measured in PAS-stained kidney sections. (E) Plasma creatinine concentration. ^***P<0.001 vs. siControl.