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. 2017 Sep 14;9(4):1043–1052. doi: 10.1016/j.stemcr.2017.08.008

Figure 1.

Figure 1

Differentiation of hPSCs into Neural Crest

(A) Schematic of the neural crest differentiation protocol. Blue, E6 medium; orange, NCN2 induction medium.

(B) hESC Shef6 and H9 (WA09) and hiPSC Miff-1 upregulate p75 after 7 days in NCN2; expression is low in undifferentiated cells.

(C) Expression of TFAP2a, PAX3, and SOX10 at day 7 (qPCR). The stem cell marker OCT4 is repressed. Gene expression is presented relative to initial undifferentiated hPSCs (RQ). Error bars represent SD. Data for each cell line is from a single experiment representing the potential differentiation efficiency obtainable. Pooled data were not used because of reproducibility issues when using NCN2 alone as described and addressed below.