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. 2017 Sep 19;7(9):e1235. doi: 10.1038/tp.2017.208

Figure 3.

Figure 3

Fluoxetine treatment shifts maturational changes in the expression of DCX and calbindin in new neurons. The experimental time line is described in Figure 1a. (a) Representative images of two GFP+/Prox1+ cells each from the vehicle- and fluoxetine-treated mice on day 28. One of them expresses DCX. Red: DCX, Cyan: Prox1, Green: GFP. (b) Representative images of two GFP+/Prox1+ cells each from the vehicle- and fluoxetine-treated mice on day 28. Three of them express calbindin. Red: calbindin, Cyan: Prox1, Green: GFP. (c, d) Percentages of DCX+ cells in GFP+/Prox1+ cells (c) and calbindin+ cells in GFP+/Prox1+ cells (d) on days 14, 21, and 28. *P<0.05, Mann–Whitney U test with Bonaferroni correction. (e) Representative images of the granule cell layer from vehicle- and fluoxetine-treated mice. Red: DCX, Cyan: Prox1. (f) Representative images of the granule cell layer from the vehicle- and fluoxetine-treated mice. Red: calbindin, Cyan: Prox1. (g) Percentage of DCX+ cells and calbindin+ cells in Prox1+ cells on days 14, 21 and 28. Scale bars, 10 μm (a and b), 30 μm (e and f).