Figure 2.

Identification of Hematopoietic-Generating Tissue in Teratomas

(A) Representative images from GFG teratoma sections stained with H&E. These tissues contain a large number of endothelial-like cells (ECs), annotated with black arrows. Hematopoietic cells (HCs) appearing to directly bud from ECs are annotated with white arrows.

(B) Representative images from GFG iPSC-derived teratoma sections stained with Runx1 and CD31. Runx1⁺ cells were detected by DAB (brown color) and CD31⁺ were detected by Bajoran purple (Purple color), with sites of double staining identified as putative sites of hematopoietic cell emergence, as marked by white arrows.

(C) Representative images of teratoma tissue clearing in chemical cocktails and computational analysis (CUBIC); non-cleared on the left, cleared on the right.

(D) Localization of eR1⁺ cells (green) and DAPI (blue) in 2D reconstructed image between eR1-derived and eR1-GFG-derived teratoma.

(E) Localization of eR1⁺ cells (green) and DAPI (blue) in 3D reconstructed images between eR1-derived and eR1-GFG-derived teratoma.

(F) Representative flow cytometric plots for CD45 and GFP expression in the teratoma from eR1-EGFP iPSC-derived teratomas.

(G) Percentage GFP⁺CD45⁺ cells in teratomas, using gating displayed in (F) (n = 3). ^∗p < 0.05.