Figure 1.

SMMC7721 mRNA expression and cell viability after 24 h treatment with different doses of pterostilbene (PTER). (A) Cells were treated with DMSO [control (Ctrl)] or PTER (5, 25, 50, or 100 µM) for 24 h. Cells were harvested, and the abundance of metastasis-associated protein 1 (MTA1) mRNA transcripts was measured. Results were analyzed by real-time PCR and calculated using the equation 2^−ΔΔcq. The ΔCq of MTA1 mRNA transcripts was calculated by subtracting the Ct of β-actin from that of MTA1; then, the ΔΔCq equal to the ΔCq from the PTER-treated groups was subtracted from that of the ΔCq Ctrl group. (B) Cells were treated with DMSO (Ctrl) or PTER (5, 25, 50, or 100 µM) for 24 h to detect cell viability. The Ctrl was normalized to 1, and cell viability values were calculated relative to those of Ctrl. Values represent the means ± standard deviation from three independent experiments. Comparisons between groups were performed using unpaired two-tailed Student's t-test; *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.