Figure 2.

Inhibition of the metastasis-associated protein 1 (MTA1)-histone deacetylase (HDAC) complex by pterostilbene, which induced the downregulation of MTA1, HDAC1 and HDAC2 expression in pterostilbene (PTER)-treated cells. (A) SMMC7721 cells were treated with various concentrations (5, 25, 50 or 100 µM) of PTER for 24 h; the control (Ctrl) group was treated with DMSO. Protein expression levels were analyzed by western blotting for MTA1, HDAC1, HDAC2 and β-actin. (B-D) Western blotting results were quantified using Image J analysis software. The means ± standard error of three independent expression are shown; **P<0.01, ***P<0.001, ****P<0.0001.