Figure 4.

Real-Time Clonal Evolution in Confetti Mouse Corneas

(A–F) Intra-vital microscopy of Confetti mice following a chase period of 3 (A), 8 (B), 20 (C), 32 (D), 40 (E) and 60 (F) weeks. Expression of RFP (first column), CFP (second column), YFP (third column), and merged images (last column) are displayed. Hatched circle represents the outline of the intra-ocular lens. RFP, red fluorescent protein; CFP, cerulean fluorescent protein; YFP, yellow fluorescent protein.

(G) Fluorescent streak number (n = 6 corneas/time point, ± SD, ^∗∗p < 0.01, ^∗∗∗p < 0.001, and ^∗∗∗∗p < 0.0001, one-way ANOVA with Holm-Sidak multiple comparison test).

(H) Fluorescent streak width (n = 6 corneas/time point, ± SD, ^∗∗∗∗p < 0.0001, one-way ANOVA with Holm-Sidak multiple comparison test).

(I) The width of the fluorescent streaks (in number of cells) against the inverse square root of the appropriate time point. Streak width was transformed from micrometers to number of cells based on the average width of a corneal epithelial basal cell of 10 μm. Data points represent the mean (n = 3 corneas/time point, ± SD, linear regression).

Scale bars: (A–F) 400 μm. See also Figure S3.