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Analysis of protein aggregation using SDD-AGE. Protein from 1-day-old adult C. elegans expressing GFP-HttQ28 and GFP-HttQ74 and third-instar D. melanogaster larvae expressing HttQ25-eGFP, HttQ46-eGFP, HttQ72-eGFP, and HttQ97-eGFP were electrophoresed on an SDD-AGE gel and detected using an anti-GFP antibody. Sample loading is based on total protein determination: 5 μg for HttQ28, 2 μg for HttQ74, and 2 μg each for HttQ25, HttQ46, HttQ72, and HttQ97.
