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. Author manuscript; available in PMC: 2018 Sep 5.
Published in final edited form as: Biochemistry. 2017 Aug 24;56(35):4676–4688. doi: 10.1021/acs.biochem.7b00518

Figure 3.

Figure 3

MSM–WDA analysis of (A) HttQ25-eGFP, HttQ46-eGFP, HttQ72-eGFP, and HttQ97-eGFP in D. melanogaster and (B) GFP-HttQ28 and GFP-HttQ74 in C. elegans. All samples prepared in lysis buffer were centrifuged at 3000, 6000, 10000, 20000, 30000, and 50000 rpm until the meniscus was cleared. The log plot of s*g(s*) vs s* shows the complete distribution of s values ranging from 0.8 to 3000 S, and the inset focuses on the 20–3000 S region to better highlight the distribution of the intermediate aggregates.