Figure 4.

Protection by HNG from neuron apoptosis in OGD/R is related to Jak2/Stat3 signaling. SH-SY5Y cells were subjected to OGD/R processes following the procedures of culture in medium with deprivation of glucose and serum in a hypoxic condition (0.5% O₂) for 16 h, and then incubation in normoxic conditions in normal medium for an additional 9 h. In OGD/R processes, the cells were treated with 1 µg/l HNG alone or 1 µg/l HNG and 5 µM FLLL32 in combination. FLLL32 is a specific inhibitor of Jak2/Stat3 signaling. (A) Cell viability, (B) apoptosis rate and (C) western blot assays were undertaken following OGD/R treatments. *P<0.05 vs. control; ^#P<0.05 vs. 0 µg/l HNG OGD/R group; ^&P<0.05 vs. OGD/R + 1 µg/l HNG group. HNG, S14G-humanin; OGD/R, oxygen glucose deprivation/reoxygenation; Jak2, Janus kinase 2; Stat3, signal transducer and activator of transcription 3; FITC, fluorescein isothiocyanate; Bax, B-cell lymphoma 2-associated X protein.