Figure 4.

T cell proliferation induced by MUC1-CRT-infected dendritic cells. Splenocytes were incubated with adenovirus-infected dendritic cells and CD3⁺/CD4⁺/CD8⁺ T cells were detected by flow cytometry using anti-CD3-PerCP, anti-CD4-Pacific Blue and anti-CD8-Alexa Flour 700 antibodies. (A and B) Control, (C and D) pEGFP-MUC1 and (E and F) pEGFP-MUC1-CRT. A, C and E, CD3 T cells; B, D and F, CD4 and CD8 T cells. CD, cluster of differentiation; MUC1, mucin; PerCP, peridinin chlorophyll protein complex; CRT, calreticulin.