Figure 5.

IFN-γ and TNF-α production by MUC1-CRT-infected dendritic cells. Dendritic cells were infected with adenoviruses containing MUC1 and MUC1-CRT, respectively, and further co-incubated with lymphocytes. Non-treated cells were used as control, and cells treated with mock vehicle were used as Nco. Culture supernatants were used to analyze (A) IFN-γ and (B) TNF-α levels by ELISA assay. Data are presented as the mean ± standard deviation (n=3). *P<0.05 and **P<0.005. INF-γ, interferon-γ; TNF-α, tumor necrosis factor-α; MUC1, mucin 1; CRT, calreticulin; Nco, null control.