Figure 1.

WT1 Increases in Renin-Stained Cells in the Juxta-Glomerular Compartment Following Podocyte Depletion in the Cytotoxic Anti-podocyte Antibody Model of FSGS

Confocal microscopy images marked (A–E) show the merge for WT1 (green), renin (red), collagen IV (blue), and DAPI (gray). Collagen IV demarcates glomeruli. Insets show the JGC for each channel, labeled as superscripts. Scale bars, 20 μm.

(A) Baseline (n = 5), WT1 (A1) is limited to podocytes and not detected in renin-stained cells (A2).

(B) D3 (n = 5), WT1 (B1) is detected in the cytoplasm of renin-stained cells (B2).

(C) D5 (n = 5), WT1 (C1) increases in JGC, overlapping with renin (C2), creating a yellow color.

(D) D14 (n = 5), renin-stained cells (D2) co-staining with WT1 (D1) increase in the JGC.

(E) D28 (n = 8), WT1 (E1) and renin (E2) co-staining persists.

(F) Quantitation of the percentage of renin-stained cells in the JGC co-staining for WT1.