Figure 3.

WT1 Does Not Increase in CoRL in RenWt1^fl/fl Mice Following Podocyte Depletion in the Cytotoxic Anti-podocyte Antibody Model of FSGS

Confocal microscopy for WT1 (green), RFP (red), collagen IV (blue), and DAPI (gray) in FSGS in RenWt1^+/+ (A–C) and RenWt1^fl/fl (D–F) mice. Insets show the JGC for each channel, labeled as superscripts. Scale bars, 20 μm.

(A–C) RenWt1^+/+ mice. (A) At baseline WT1 is limited to podocytes (A1); RFP is limited to the JGC (A2). (B) At D28, WT1 (B1) merges with RFP (B2) in the JGC, creating a yellow color. WT1 is reduced in glomeruli. (C) At D28, after 25 days of enalapril, WT1 (C1) and RFP (C2) are increased in the JGC.

(D–F) RenWt1^fl/fl mice. (D) At baseline, WT1 (D1) is limited to podocytes; RFP (D2) is limited to JGC. (E) At D28, WT1 is reduced in podocytes, and not detected (E1) in RFP⁺ cells (E2) in the JGC. (F) At D28, after 25 days of enalapril, there is no WT1(F1) in RFP⁺ cells (F2).

(G) Stacked bar graph shows the number of RFP⁺CoRL not expressing WT⁻ (red) and co-expressing WT1⁺ (green) in the JGC. RFP⁺WT1⁺ cells increase progressively in diseased RenWt1^+/+ but not in RenWt1^fl/fl mice (n = 13/group).