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. 2017 Sep 28;9(4):1152–1166. doi: 10.1016/j.stemcr.2017.08.020

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© 2017 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

RFP⁺CoRL MET Is Significantly Reduced in Diseased RenWt1^fl/fl Mice Compared with Diseased RenWt1^+/+ Mice

Confocal microscopy for myosin11 (MYH11, green, mesenchymal marker), RFP (red, CoRL), and DAPI (blue, nuclei) in the JGC (A–F). Insets show each channel, labeled as superscripts. Scale bars, 20 μm.

(A–C) RenWt1^+/+ mice. (A) At baseline, MYH11 (A1) merges with RFP (A2) in the JGC, creating a yellow color. (B) At FSGS D28, MYH11 (B1) decreases in RFP⁺ (B2) cells. (C) At D28 after enalapril, MYH11 (C1) decreases, despite an increase in RFP⁺ cells (C2).

(D–F) RenWt1^fl/fl mice. (D) At baseline, MYH11 (D1) merges with RFP (D2). (E) At FSGS D28, MYH11 (E1) in RFP⁺ cells (E2, solid arrows) persists. (F) At D28 after enalapril, MYH11 (F1) in RFP⁺ cells (F2, solid arrows) also persists.

(G) The percentage of RFP⁺CoRL expressing MYH11 decreases in RenWt1^+/+ mice with FSGS and is augmented by enalapril. RenWt1^fl/fl mice show no significant decrease in MYH11 (n = 13/group).

(H–M) Confocal microscopy for cytokeratin 18 (CK18, green, epithelial marker), RFP (red, CoRL), and DAPI (blue, nuclei) in the JGC (H–M). Insets show each channel, labeled as superscripts. Scale bars, 20 μm.

(H–J) RenWt1^+/+ mice. (H) At baseline, CK18 (H1) is barely detected in RFP⁺ cells (H2). (I) At FSGS D28, CK18 (I1) increases in RFP⁺ cells (I2), creating a yellow color (solid arrows). (J) At D28 after enalapril, CK18 (J1) further increases in RFP⁺ cells (J2), creating a yellow color (solid arrows).

(K–M) RenWt1^fl/fl mice. (K) At baseline, CK18 (K1) is not detected in RFP⁺ cells (K2). At FSGS D28 (L) and after enalapril (M), CK18 (L1, M1) does not increase in RFP⁺ cells (L2, M2).

(N) The percentage of RFP⁺CK18⁺cells increases in diseased RenWt1^+/+ mice, which is augmented by enalapril. CK18 does not increase significantly in diseased RenWt1^fl/fl mice (n = 13/group).