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. 2017 Sep 28;9(4):1304–1314. doi: 10.1016/j.stemcr.2017.08.022

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© 2017 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Screening for Pluripotency-Related Candidates

(A) Schematic depicting the screening procedure.

(B) Fluorescent (top) and phase-contrast (bottom) images showing changes in the YFP-tagged protein levels before (left) and after (right) 48 hr of RA-induced differentiation. Scale bar, 100 μm. LIF, leukemia inhibitory factor.

(C and D) Western blot analyses using anti-GFP antibodies (C) and qPCR (D) of the selected clones shown in (B) before and after 48 hr of RA-induced differentiation. Protein and mRNA levels were normalized to GAPDH protein and mRNA, respectively. Data were obtained from 3 independent experiments; error bars represent ±SD.