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. 2017 Oct 12;14:201. doi: 10.1186/s12974-017-0975-6

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — ASH suppressed inflammation caused by DIO in the hypothalamus. a, b Representative Western blot analysis for Iba1, TNFα, and IL-1β in the hypothalamus region of LFD, HFD, and HFDE animals. Histograms represent percent change in intensity taking value in LFD rats as 100%. c Histograms representing fold change in mRNA expression of iNOS, COX2, TNFα, and IL-1β in the hypothalamus region of rat brain among the four groups of animals (n = 3–4 each group). Values are expressed as mean ± SEM. * p ≤ 0.05 LFD versus HFD, LFDE, and HFDE rats, ^# p ≤ 0.05 HFD versus HFDE rats, ^$ p ≤ 0.05 HFD versus LFDE rats, ^@ p ≤ 0.05 LFDE versus HFDE rats, Holm–Sidak method after one-way ANOVA