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Electrophoretic analysis of nucleic acids from intracellular fractions of E. coli DH5α (A) and R. sulfidophilum (B), both containing the plasmid pHBSR1-RM-pre-miR-29b. Samples were recovered from cell suspensions at different periods of fermentation and the total RNA fractions were obtained by the phenol-chloroform method using TripleXtractor reagent (GRISP-Research Solutions, Porto, Portugal). Lanes, cultivation periods. Lane M, molecular weight marker.
