Table 1.
Strains and vectors used in this study.
| Strains or vectors | Properties or product | Reference or source |
|---|---|---|
| E. coli TOP10F’ | F’ [lacIq Tn10(Tetr)] mcrA (mrr-hsdRMS-mcrBC) 80lacZM15 lacX74 recA1araD139 (ara-leu)7697 galU galK rpsL (Strr) endA1 nupG | Invitrogen, Carlsbad, CA |
| S. cerevisiae (BCRC 21685) (SC) | Wild type | Bioresources Collection and Research Center, Taiwan |
| Sacchromyces cerevisiae with overexpression of tps1 gene (SCT) | Carrying pGAPZC-tps1 vector | Divate et al., 2016 |
| Sacchromyces cerevisiae with overexpression of tps1 and ari1 gene (SCTA) | Carrying pGAPZC-tps1 vector and pGAPZC-ari1 vector | This study |
| Sacchromyces cerevisiae with overexpression of tps1 gene and deletion of nth1 gene (SCTΔN) | Carrying pGAPZC-tps1 vector and gene disruption cassette | Divate et al., 2016 |
| Sacchromyces cerevisiae with overexpression of tps1, ari1 genes and deletion of nth1 gene (SCTAΔN) | Carrying pGAPZC-tps1 vector, pGAPZC-ari1 vector and gene disruption cassette | This study |
| pGAPZαC | Expression Vector, GAP promoter, Sh ble gene (Zeocin™ resistance gene) | Invitrogen, Carlsbad, CA |
| pGAPZC-ari1 | pGAPZαC vector carrying a ari1 gene | This study |